Description

Violet Red Bile Agar (VRB) 500gm Himedia

Violet Red Bile Agar (VRBA) is a selective medium recommended for the isolation, detection, and enumeration of coliform bacteria, particularly in dairy products, water, and other food samples. The medium inhibits the growth of gram-positive organisms through the presence of bile salts and crystal violet, making it ideal for selective cultivation of gram-negative enteric bacteria, especially coliforms like Escherichia coli.

Composition (per liter):

• Peptone – 7.0 g

• Yeast Extract – 3.0 g

• Bile Salts – 1.5 g

• Lactose – 10.0 g

• Sodium Chloride – 5.0 g

• Neutral Red – 0.03 g

• Crystal Violet – 0.002 g

• Agar – 15.0 g

• Final pH (at 25°C): 7.4 ± 0.2

Uses:

• Detection and enumeration of coliforms in:

  • Milk and dairy products

  • Food and beverages

  • Water and wastewater

• Quality control testing in food microbiology laboratories

• Routine microbial monitoring in pharmaceutical and cosmetic industries

Features:

• Selective Medium: Inhibits gram-positive organisms using bile salts and crystal violet.

• Differential Medium: Contains lactose and neutral red to differentiate lactose fermenters (which appear red to pink colonies) from non-fermenters.

• Reliable and standard: Conforms to ISO and APHA methods for coliform detection.

• High Clarity and Performance: Ensures reproducible results with high colony visibility.

• Suitable for Pour Plate or Surface Spreading Methods